Alkaline butanol extraction of bile salt and steroid sulfate esters: Application to the assay of sulfotransferases

Academic Article


  • A butan-1-ol solvent-extraction procedure has been evaluated for the assay of 3′-phosphoadenosine-5′-phosphosulfate:sulfotransferase activity with various bile salt and steroid substrates Although butanol extracted the sulfate esters of steroids and bile salts from aqueous solution at neutral pH, extraction at basic pH gave optimum recovery which was independent of protein in the sample. Greater than 99.9% of unreacted 3′-phosphoadenosine-5′-phospho[35S]sulfate remained in the aqueous phase. The data for sulfotransferase activities obtained with this solvent-extraction assay were not significantly different from those obtained with a standard thin-layer chromatography method. Solvent extraction has enabled multiple, rapid assays of several steroid and bile salt sulfotransferases during chromatographic purification of these enzymes from tissue fractions. © 1983.
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    Digital Object Identifier (doi)

    Author List

  • Barnes S; Waldrop R; Neighbors AS
  • Start Page

  • 470
  • End Page

  • 475
  • Volume

  • 133
  • Issue

  • 2