Lipid-exchange in nanodiscs discloses membrane boundaries of cytochrome-P450 reductase

Academic Article


  • Lipids are critical for the function of membrane proteins. NADPH-cytochrome-P450-reductase, the sole electron transferase for microsomal oxygenases, possesses a conformational dynamics entwined with its topology. Here, we use peptide-nanodiscs to unveil cytochrome-P450-reductase's lipid boundaries, demonstrating a protein-driven enrichment of ethanolamine lipids (by 25%) which ameliorates by 3-fold CPR's electron-transfer ability.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Barnaba C; Ravula T; Medina-Meza IG; Im SC; Anantharamaiah GM; Waskell L; Ramamoorthy A
  • Start Page

  • 6336
  • End Page

  • 6339
  • Volume

  • 54
  • Issue

  • 49