Repression of glucocorticoid receptor gene transcription by c-Jun

Academic Article

Abstract

  • The regulation of glucocorticoid receptor gene expression by members of the AP-1 family was examined in glucocorticoid-free NIH3T3 cells transfected with the human glucocorticoid receptor gene promoter driving expression of a CAT reporter gene. c-Jun inhibited the promoter activity by 80% and JunB by 30%, whereas c-Fos and JunD had no inhibitory effect. Electrophoretic mobility shift assays showed that c-Jun is unable to efficiently interact with the AP-1-like site present in the human glucocorticoid receptor promoter. Moreover, c-Jun was still able to repress promoter mutants in which the region containing the AP-1-like site was deleted. NIH3T3 cell clones overexpressing c-Jun exhibited lower glucocorticoid receptor mRNA levels, which suggests that the murine glucocorticoid receptor gene can also be regulated by AP-1. These results provide a new mechanism for cross-talk between the glucocorticoid receptor and the AP-1 family of transcription factors in the absence of glucocorticoid ligands. Copyright © 2001 Elsevier Science Ireland Ltd.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 13583939
  • Author List

  • Cabral ALB; Hays AN; Housley PR; Brentani MM; Martins VR
  • Start Page

  • 67
  • End Page

  • 79
  • Volume

  • 175
  • Issue

  • 1-2