Inhibition of human anti‐αGal IgG by oligosaccharides derived from porcine stomach mucin

Academic Article

Abstract

  • Abstract: We now report that neutral oligosaccharides derived from porcine stomach mucin (PSM) are potent inhibitors of human anti‐αGal antibodies, which are the major cause of hyperacute vascular rejection following discordant organ xenotransplantation. The O‐linked oligosaccharides of PSM were released through β‐elimination, and neutral oligosaccharides were obtained. In ELISA these oligosaccharides inhibited the binding of purified anti‐αGal IgG to mouse laminin, α glycoprotein known to contain α‐galactosyl determinants. Treatment of oligosaccharides with a‐galactosidase, but not β‐galactosidase, abolished their inhibitory activity. The oligosaccharides were size‐fractionated on a column of Bio‐Gel P‐6, and it was found that the larger oligosaccharides were more potent than the smaller in inhibiting the anti‐αGal IgG. Less than 1 % of the total oligosaccharides from PSM bound to a column containing immobilized anti‐αGal IgG. These bound oligosaccharides were nearly 1,000‐fold more potent than the unbound oligosaccharides in inhibiting anti‐αGal antibody. It may be possible to overcome the hyperacute vascular rejection of discordant organ xenotransplants by using these affinity‐purified oligosaccharides from PSM in immunoaffinity columns or as a continuous intravenous infusion. © 1995 Munksgaard
  • Authors

    Digital Object Identifier (doi)

    Pubmed Id

  • 22667658
  • Author List

  • Li S; Yeh J; Cooper DKC; Cummings RD
  • Start Page

  • 279
  • End Page

  • 288
  • Volume

  • 2
  • Issue

  • 4