Binding and specificity of major immunoglobulin classes of preformed human anti-pig heart antibodies

Academic Article

Abstract

  • Preformed human anti-pig antibodies isolated from perfused pig hearts were used to analyze the binding of various immunoglobulin classes to cultured pig kidney cells. All anti-pig immunoglobulins (i.e., IgG, IgA, and IgM) were localized on the cell surface by the use of an indirect immunofluorescence technique. Anti-pig immunoglobulins also competed for the pig cell surface epitopes with Griffonia simplicifolia lectin (GS-I-B4), which is specific for α-galactosyl residues. This study provides further evidence that preformed human antibodies recognizing α-glactosyl-containing epitopes (anti-gal antibodies) could be an important factor in hyperacute rejection of pig organs. © 1993 Springer-Verlag.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Koren E; Neethling FA; Richards S; Koscec M; Ye Y; Zuhdi N; Cooper DKC
  • Start Page

  • 351
  • End Page

  • 353
  • Volume

  • 6
  • Issue

  • 6