The development and characterization of encephalitogenic cloned T cells specific for myelin proteolipid protein

Academic Article

Abstract

  • T-cell clones have been isolated from SJL/J mice after immunization with myelin proteolipid protein (PLP). The cloned cells responded strongly to PLP stimulation in vitro as well as to the synthetic PLP-related peptide 139-151. The response to PLP is Ia mediated, since it was inhibited by monoclonal antibodies to the matched I-As haplotype, but not with antibodies to other I-A haplotypes. Phenotypic analysis using immunofluorescence demonstrated the following characteristics of the clones: Thy-1+, CD4+, CD5+ and CD8-. Injection of 10-30 million PLP-activated cells from one clone induced severe experimental allergic encephalomyelitis in five mice, both clinically and histologically. This represents to our knowledge the first report of PLP-specific encephalitogenic cloned T cells. © 1990.
  • Authors

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    Digital Object Identifier (doi)

    Author List

  • van der Veen RC; Trotter JL; Hickey WF; Kapp JA
  • Start Page

  • 139
  • End Page

  • 145
  • Volume

  • 26
  • Issue

  • 2