Repigmentation of human retinal pigment epithelial cells in vitro

Academic Article

Abstract

  • Cultured human retinal pigment epithelial (RPE) cells readily ingested both melanin and lipofuscin isolated from human RPE cells. Up to 7 days post-challenge ingested granules demonstrated no evidence of lysis or aggregation within secondary lysosomes. When cultures containing ingested melanin and lipofuscin were subcultured the cells gradually depigmented due to a redistribution of pigment granules amongst daughter cells. Quantitative analysis demonstrated that the accumulation of both types of granule was linear over a 24 hr challenge period. This study reports a technique of 'artificially' repigmenting cultured human RPE cells and thus offers the potential for in vitro investigations of the role of these inclusions in various dynamic aspects of cellular metabolism. © 1985.
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    Digital Object Identifier (doi)

    Author List

  • Boulton M; Marshall J
  • Start Page

  • 209
  • End Page

  • 218
  • Volume

  • 41
  • Issue

  • 2