Retinoylation of cytokeratins in normal human epidermal keratinocytes

Academic Article


  • Retinoylation (retinoic acid acylation) is a covalent modification of proteins occurring in a variety of eukaryotic cell lines. In this study, we found that proteins in undifferentiated and squamous-differentiated normal human epidermal keratinocytes were retinoylated after treatment with [3h]retinoic acid. The major retinoylated proteins were identified as cytokeratins based on their profile in two-dimensional gel electrophoresis and their immunoreactivity with anti-keratin monoclonal antibodies. The covalently bound [3h]retinoic acid was not removed by mild hydrolysis with methanolic-KOH indicating that it is not linked to the cytokeratins by a thioester bond. The results raise the possibility that retinoylation of cytokeratins is involved in some of the effects of retinoic acid on keratinocytes. © 1991 Academic Press, Inc.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 9018226
  • Author List

  • Takahashi N; Jetten AM; Breitman TR
  • Start Page

  • 393
  • End Page

  • 400
  • Volume

  • 180
  • Issue

  • 1