The Krüppel-like zinc finger protein Glis2 functions as a negative modulator of the Wnt/β-catenin signaling pathway

Academic Article

Abstract

  • To gain insight into the mechanism by which Gli-similar 2 (Glis2) regulates transcription, we performed yeast-two hybrid cDNA library screening using Glis2 as bait. This screening identified β-catenin as a potential Glis2-interacting protein. Mammalian two-hybrid, co-immunoprecipitation, and GST-pulldown analyses supported the interaction between Glis2 and β-catenin. Pulldown analyses with several Glis2 deletion mutants indicated that the 1st zinc finger motif of Glis2 is critical for its interaction with β-catenin, while the armadillo repeats of β-catenin are important in its interaction with Glis2. Reporter analyses showed that Glis2 represses T-cell factor (TCF)-mediated transcriptional activation. In addition, Glis2 represses the expression of the TCF target gene cyclin D1. Our results indicate that Glis2 interacts with β-catenin and suggest that Glis2 functions as a negative modulator of β-catenin/TCF-mediated transcription.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 9349284
  • Author List

  • Kim YS; Kang HS; Jetten AM
  • Start Page

  • 858
  • End Page

  • 864
  • Volume

  • 581
  • Issue

  • 5