FoxO1 represses LXRα-mediated transcriptional activity of SREBP-1c promoter in HepG2 cells.

Academic Article

Abstract

  • Recent studies have demonstrated that FoxO1 modulates the expression of SREBP-1c, but the exact mechanism remains unknown. Our results demonstrate that FoxO1 suppresses the SREBP-1c promoter transcriptional activity in HepG2 cells. This repression was independent of FoxO1 binding to the SREBP-1c promoter, but LXR responsive elements (LXREs) were crucial to this phenomenon. Moreover, FoxO1 also strongly inhibited the LXRα-mediated elevated transcription by SREBP-1c promoter. Electrophoretic mobility shift assay and chromatin immuno-precipitation further suggested the ability of FoxO1 to inhibit LXRα binding with the LXRE in the SREBP-1c promoter. FoxO1-mediated suppression of SREBP-1c promoter activity could be partially alleviated by insulin.
  • Authors

    Keywords

  • Animals, Blotting, Western, CHO Cells, Chromatin Immunoprecipitation, Cricetinae, Cricetulus, Forkhead Box Protein O1, Forkhead Transcription Factors, Hep G2 Cells, Humans, Insulin, Liver X Receptors, Luciferases, Mice, Mutation, Orphan Nuclear Receptors, Promoter Regions, Genetic, Protein Binding, Response Elements, Reverse Transcriptase Polymerase Chain Reaction, Sterol Regulatory Element Binding Protein 1, Transcriptional Activation, Transfection
  • Digital Object Identifier (doi)

    Pubmed Id

  • 25315148
  • Author List

  • Liu X; Qiao A; Ke Y; Kong X; Liang J; Wang R; Ouyang X; Zuo J; Chang Y; Fang F
  • Start Page

  • 4330
  • End Page

  • 4334
  • Volume

  • 584
  • Issue

  • 20