Immunofluorescence on pronase-digested paraffin sections: a valuable salvage technique for renal biopsies.

Academic Article


  • Direct immunofluorescence (IF) on frozen tissue is the method of choice for the study of medical renal diseases. When no glomeruli are available, IF can be performed on the formalin-fixed paraffin-embedded tissue allocated for light microscopy after antigen retrieval with proteases. In this study, the results of IF on frozen tissue (IF-F) and on deparaffinized, pronase-treated tissue (IF-P) were compared in 71 renal biopsies representing 12 major renal diseases. Using IF-P, diagnostic findings were obtained in 100% of cases of lupus nephritis, acute post-infectious glomerulonephritis, cryoglobulinemic glomerulonephritis, fibrillary glomerulonephritis, primary amyloidosis, myeloma cast nephropathy, and light-chain Fanconi syndrome (LCFS), 88% of cases of immunoglobulin (Ig)A nephropathy, 80% of cases of light-chain deposition disease, 60% of cases of membranoproliferative glomerulonephritis type 1, 50% of cases of idiopathic membranous glomerulopathy (MGN) and 20% of cases of anti-glomerular basement membrane (GBM) disease. IF-P was less sensitive than IF-F for the detection of C3 in all disease categories and for the detection of IgG in cases of MGN and anti-GBM disease. The diagnostic kappa light-chain staining was demonstrated in 100% of cases of LCFS by IF-P versus 40% by IF-F. We conclude that IF-P is a valuable salvage immunohistochemical technique for renal biopsies lacking adequate cortical sampling for IF-F, and is superior to IF-F for the diagnosis of LCFS.
  • Authors

    Published In


  • Antigen-Antibody Complex, Biopsy, Fluorescent Antibody Technique, Frozen Sections, Humans, Immunoglobulin G, Immunoglobulin kappa-Chains, Kidney, Kidney Diseases, Paraffin Embedding, Pronase, Sensitivity and Specificity
  • Digital Object Identifier (doi)

    Author List

  • Nasr SH; Galgano SJ; Markowitz GS; Stokes MB; D'Agati VD
  • Start Page

  • 2148
  • End Page

  • 2151
  • Volume

  • 70
  • Issue

  • 12