A simple and rapid dot-immunobinding assay for zein and other prolamins

Academic Article


  • A simple method for the immunochemical assay of zein and other prolamins is described. Alcohol-solubilized zein is spotted onto chromatography paper disks or strips, incubated with antiserum (30-60 min), washed with several changes of Tris-buffered saline (10 min), incubated with protein A-peroxidase (30 min), and washed with several changes of Tris-buffered saline (10 min). The binding of zein-specific antibody is visualized by peroxidase-catalyzed color production from 4-chloro-1-naphthol. The method is simple, inexpensive, and rapid (2 h total time) and has potential application to other insoluble antigens. © 1983.
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    Digital Object Identifier (doi)

    Author List

  • Esen A; Conroy JM; Wang SZ
  • Start Page

  • 462
  • End Page

  • 467
  • Volume

  • 132
  • Issue

  • 2