Akt-dependent and isoform-specific regulation of dopamine transporter cell surface expression

Academic Article

Abstract

  • Dopamine (DA) is a neurotransmitter implicated in multiple functions, including movement, cognition, motivation, and reward. The DA transporter (DAT) is responsible for clearing extracellular DA, thereby terminating DA neurotransmission. Previously, it has been shown that insulin signaling through protein kinase B/Akt regulates DAT function by fine-tuning DAT cell surface expression. Importantly, specific Akt isoforms (e.g., Akt1, Akt2) serve distinct physiological functions. Here, we demonstrate using isoform-specific Akt inhibitors that basal activity of Akt2, rather than Akt1, regulates DAT cell surface expression. Since Akt2 activation is mediated by insulin, these data further implicate insulin signaling as an important modulator of DAT function and dopaminergic tone. © 2010 American Chemical Society.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 20812751
  • Author List

  • Speed NK; Matthies HJG; Kennedy JP; Vaughan RA; Javitch JA; Russo SJ; Lindsley CW; Niswender K; Galli A
  • Start Page

  • 476
  • End Page

  • 481
  • Volume

  • 1
  • Issue

  • 7