Objective: To compare the ability of 24-2 frequency-doubling perimetry (FDP-Matrix) with standard automated perimetry with the Swedish interactive threshold algorithm (SAP-SITA) in detection of visual function abnormalities in patients with glaucomatous-appearing optic discs (GAOD). Methods: This observational case-control study included 80 patients with GAOD and 54 control subjects diagnosed by masked assessment of optic disc stereoscopic photographs. Abnormal visual function at SAPSITA and FDP-Matrix testing required consistent abnormalities in 2 visual field examinations, determined using the glaucoma hemifield test outside 99% normal limits, pattern standard deviation outside 95% normal limits, or 3 contiguous points in the pattern deviation probability plot outside 95% normal limits (at least 1 P<1%) within the same hemifield. Results: The FDP-Matrix and SAP-SITA detected abnormal visual function in 51% and 44%, respectively, of GAODeyes (P=.26), and both perimetry techniques identified 11% of healthy eyes as abnormal. Agreement between FDP-Matrix and SAP-SITA was moderate (κ=0.49), as only 35% of GAOD eyes and 2% of healthy eyes had both visual field test results flagged as abnormal. Conclusions: The FDP-Matrix detected abnormal visual function in more eyes with GAOD than did SAPSITA, although this difference was not significant. Each visual field test tended to identify different subsets of eyes with GAOD as abnormal. Combination of these perimetry techniques may improve the detection of visual function abnormalities in patients with glaucoma. ©2007 American Medical Association. All rights reserved.