Comparison of morphology of human macular and peripheral Bruch's membrane in older eyes

Academic Article


  • Deposits in macular human Bruch's membrane (BrM) increase with age and have been postulated to be associated with age-related maculopathy. We used two ultrastructural methods to compare these deposits by electron microscopy in macular and peripheral BrM of eight eyes from donors 63-86 years of age. Quick-freeze/deep-etch (QFDE) was used to prepare replicas that showed the ultrastructure of deposits, and osmium-tannic acid-paraphenylenediamine (OTAP) was used to preserve small extracellular lipid particles. We found that an accumulation of lipoprotein-like particles (LLPs) occurred in the peripheral BrM just as it does in the macular region, but with perhaps a somewhat slower time course. The "lipid wall," reported in macular BrM, was also found occasionally in the peripheral regions. The same processes that lead to age-related accumulation of LLPs in macular BrM appear to also occur in the peripheral regions. Copyright © Informa Healthcare USA, Inc.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Johnson M; Dabholkar A; Huang JD; Presley JB; Chimento MF; Curcio CA
  • Start Page

  • 791
  • End Page

  • 799
  • Volume

  • 32
  • Issue

  • 9