Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis

Academic Article


  • The heterotrimeric G-protein complex is minimally composed of G ±, G 2, and G 3 subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification. © 2011 EMBO and Macmillan Publishers Limited All rights reserved.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Klopffleisch K; Phan N; Augustin K; Bayne RS; Booker KS; Botella JR; Carpita NC; Carr T; Chen JG; Cooke TR
  • Volume

  • 7