A novel method of transition metal (TM)-doped II-VI nanocrystal production by laser ablation in a liquid environment is presented. The method enables fabrication of II-VI nanocrystals doped with a variety of TM ions using laser ablation of thermodiffusion-doped polycrystalline targets. The crystalline grain size and structure of nanocrystals was studied by XRD using angle mode of diffractometer with a Cu-K-alpha anode. The method has a significant advantage over chemical methods because of its simplicity and the absence of residual organic molecules on the surface of the nanoparticles. TM-doped II-VI nanocrystals demonstrated strong mid-IR luminescence. This technology also opens new pathways for future developments of optically and electrically pumped mid-IR lasers based upon TM-doped quantum confined structures.