Development and validation of an HPLC method for quantitation of BA-TPQ, a novel iminoquinone anticancer agent, and an initial pharmacokinetic study in mice

Academic Article

Abstract

  • We herein describe the development and validation of an HPLC method for the quantitation of 7-(benzylamino)-1,3,4,8-tetrahydropyrrolo [4,3,2-de]quinolin-8(1H)-one (BA-TPQ), a newly synthesized iminoquinone anticancer agent. BA-TPQ was extracted from plasma and tissue samples by first precipitating proteins with acetonitrile followed by a liquid-liquid extraction with ethyl acetate. Chromatographic separation was carried out using a gradient flow rate on a Zorbax SB C18 column, and the effluent was monitored by UV detection at 346nm. The method was found to be precise, accurate, and specific, with a linear range of 3.91-1955.0ng/mL in plasma, 19.55-1955.0ng/mL in spleen, brain, and liver homogenates and 19.55-3910.0ng/mL in heart, lung and kidney homogenates. The method was stable under all relevant conditions. Using this method, we also carried out an initial study determining plasma pharmacokinetics and tissue distribution of BA-TPQ in mice following intravenous administration. In summary, this simple and sensitive HPLC method can be used in future preclinical and clinical studies of BA-TPQ. © 2010 John Wiley & Sons, Ltd.
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    Digital Object Identifier (doi)

    Author List

  • Li H; Ezell SJ; Zhang X; Wang W; Xu H; Rayburn ER; Zhang X; Gurpinar E; Yang X; Sommers CI
  • Start Page

  • 628
  • End Page

  • 634
  • Volume

  • 25
  • Issue

  • 5