Structure - Function relationship in the antifreeze activity of synthetic alanine - Lysine antifreeze polypeptides

Academic Article


  • Recently antifreeze proteins (AFP) have been the subject of many structure - function relationship studies regarding their antifreeze activity. Attempts have been made to elucidate the structure - function relationship by various amino acid substitutions, but to our knowledge there has been no successful from first principles design of a polypeptide that would bind to designated ice planes along a specific direction. In this paper we show the results of our first attempt on an entirely de novo design of an alanine - lysine-rich antifreeze polypeptide This 43 residue alanine - lysine peptide exhibits characteristic nonequilibrium freezing point depression and binds to the designated (21̄0) planes of ice along the [122] vector. The structural and thermodynamic properties of this polypeptide were determined using circular dichroism spectroscopy and its nonequilibrium antifreeze properties were investigated using an ice-etching method and nanoliter osmometry.
  • Published In

  • Biomacromolecules  Journal
  • Digital Object Identifier (doi)

    Author List

  • Wierzbicki A; Knight CA; Rutland TJ; Muccio DD; Pybus BS; Sikes CS
  • Start Page

  • 268
  • End Page

  • 274
  • Volume

  • 1
  • Issue

  • 2