Conformationally defined retinoic acid analogues. 4. Potential new agents for acute promyelocytic and juvenile myelomonocytic leukemias

Academic Article

Abstract

  • We recently synthesized several conformationally constrained retinoic acid (RA) analogues [8-(2'cyclohexen-1'-ylidene)-3,7-dimethyl-2,4,6- octatrienoic acids with different alkyl substituents at 2' (R1) and 3' (R2) positions on the cyclohexene ring] (Muccio et al. J. Med. Chem. 1996, 39, 3625) as cancer chemopreventive agents. UAB8 (R1 = Et; R2 = (i)Pr), which contains sufficient steric bulk at the terminal end of the polyene chain to mimic the trimethylcyclohexenyl ring of RA, displayed biological properties similar to those of RA. To explore the efficacy of this retinoid in acute promyelocytic leukemia (APL) and juvenile myelomonocytic leukemia (JMML), we evaluated UAB8 isomers in in vitro assays which measure the capacity of retinoids to inhibit aberrant myeloid colony growth from blood or bone marrow cells obtained from human JMML patients and in assays measuring the potential of retinoids to differentiate NB4 cells (an APL cell line). Both (all-E)- and (13Z)-UAB8 were 2-fold more active than RA in the NB4 cell differentiation assay; however, only (all-E)-UAB8 had comparable activity to the natural retinoids in the JMML cell assays. These results were compared to the biological effectiveness of a new retinoid, UAB30 [8-(3',4'-dihydro-1'(2'H)- naphthalen-1'-ylidene)-3,7-dimethyl-2,4,6octatrienoic acid], which had different nuclear receptor binding and transactivational properties than UAB8. Relative to (all-E)-RA and (all-E)-UAB8, (all-E)-UAB30 bound well to RARα but did not activate transcription-mediated RARα homodimers, even though it was effective in RARβ- and RARγ-mediated transactivational assays. In APL assays, this retinoid had much reduced activity and was only moderately effective in JMML assays and in cancer chemoprevention assays.
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    Author List

  • Muccio DD; Brouillette WJ; Breitman TR; Taimi M; Emanuel PD; Zhang XK; Chen GQ; Sani BP; Venepally P; Reddy L
  • Start Page

  • 1679
  • End Page

  • 1687
  • Volume

  • 41
  • Issue

  • 10