Distinct peripheral vs mucosal T-cell phenotypes in chlamydia-infected women

Academic Article

Abstract

  • © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Problem: Differences in circulating (peripheral) and mucosal T-cell phenotypes in chlamydia-infected women remain largely unknown. Method of study: Thirteen paired mononuclear cell specimens from blood and cervicovaginal lavages collected from chlamydia-infected women were stained and analyzed using ten-color cell surface flow cytometry for T-cell distribution, activation status, homing, and T helper (Th)-associated chemokine receptors (CKRs). Results: A higher proportion of genital mucosal T-cells were activated (CD38+HLA-DR+) and expressed CCR5 and Th1-associated CKR CXCR3+CCR5+ compared to peripheral T-cells, but a lower proportion of mucosal T-cells expressed homing CKR CCR7, Th-2 associated CKR CCR4, and CXCR3+CCR4+ for both T-cell subsets. Conclusion: T-cell phenotypes differed in the peripheral vs genital mucosa compartments in chlamydia-infected women. As chlamydia infects mucosal epithelial cells, the finding of a higher frequency of activated T-cells and Th-1 phenotypes in the mucosa likely reflects an adaptive immune response to infection.
  • Digital Object Identifier (doi)

    Author List

  • Ogendi BMO; Bakshi RK; Sabbaj S; Brown LD; Lee JY; Kapil R; Geisler WM
  • Volume

  • 78
  • Issue

  • 6