Study of structure-function organization of variola virus genome. III. Sequencing and analysis of the nucleotide sequence of conservative region of HindIII F, N and A genome fragments of India-1967 strain

Academic Article


  • Computer analysis of variola major virus (VAR) genomic fragment bounded by open reading frames (ORFs) D1R and A33L which is 47,961 bp long revealed 46 potential ORFs. The VAR proteins were compared with the analogous proteins of vaccinia virus strain Copenhagen. The subunit of DNA-dependent RNA polymerase, as well as the transcription factors, mRNA capping enzymes, and proteins necessary for the virion morphogenesis proved to be highly conservative within orthopoxviruses. The most pronounced differences between the VAR genome fragment under study and the corresponding vaccinia virus fragment were revealed in the vicinity of the gene encoding the A-type inclusion body protein. The possible functions of the analyzed viral proteins are discussed.
  • Published In

  • Molecular Biology  Journal
  • Author List

  • Shchelkunov SN; Resenchuk SM; Totmenin AV; Kolykhalov AA; Frolov IV; Dryga SM; Volchkov VV; Chizhikov VE; Gutorov VV; Blinov VM
  • Start Page

  • 392
  • End Page

  • 406
  • Volume

  • 28
  • Issue

  • 2