Preparation and study of recombinant strains of Venezuelan equine encephalomyelitis virus expressing the PreS2-S gene of the hepatitis B virus

Academic Article

Abstract

  • Venezuelan equine encephalomyelitis (VEE) virus-based vectors for expression of heterologous genes have been constructed using full-length cDNA copy of the interstrain recombinant virus (Trinidad Donkey and 230 strain). Gene cassettes carrying the subgenomic mRNA promoter of VEE virus and the preS2-S gene of hepatitis B virus (HBV) were inserted before or after the genes of structural viral proteins. Live virus stocks were obtained by transfection of chick embryo fibroblasts with in vitro transcribed full-length RNA. Insertions of gene cassettes before the structural region resulted in expression of HBsAg (VEHB-25 and VEHB-361 viruses), whereas insertions in the 3' region did not. Recombinant virus VEHB-25 expressed HBsAg during 5 passages in Vero cells. VEHB-25 stimulated immune response to HBsAg and was less virulent than the parental virus.
  • Published In

    Author List

  • Dryga SA; Sviatchenko VA; Mikriukova TP; Fursova EI; Kiselev NN; Goncharov AM; Frolov IV; Kolykhalov AA; Agapov EV; Netesov SV
  • Start Page

  • 100
  • End Page

  • 104
  • Volume

  • 41
  • Issue

  • 3