Cutting Edge: Endogenous IFN-β regulates survival and development of transitional B cells

Academic Article

Abstract

  • Copyright © 2017 by The American Association of Immunologists, Inc. The transitional stage of B cell development is a formative stage in the spleen where autoreactive specificities are censored as B cells gain immune competence, but the intrinsic and extrinsic factors regulating survival of transitional stage 1 (T1) B cells are unknown. We report that B cell expression of IFN-β is required for optimal survival and TLR7 responses of transitional B cells in the spleen and was overexpressed in T1 B cells from BXD2 lupus-prone mice. Single-cell gene expression analysis of B6 Ifnb+/+ versus B6 Ifnb-/-T1 B cells revealed heterogeneous expression of Ifnb in wild-type B cells and distinct gene expression patterns associated with endogenous IFN-b. Single-cell analysis of BXD2 T1 B cells revealed that Ifnb is expressed in early T1 B cell development with subsequent upregulation of Tlr7 and Ifna1. Together, these data suggest that T1 B cell expression of IFN-β plays a key role in regulating responsiveness to external factors.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Hamilton JA; Wu Q; Yang P; Luo B; Liu S; Hong H; Li J; Walter MR; Fish EN; Hsu HC
  • Start Page

  • 2618
  • End Page

  • 2623
  • Volume

  • 199
  • Issue

  • 8