α-Latrotoxin stimulates glutamate release from cortical astrocytes in cell culture

Academic Article

Abstract

  • The mechanism responsible for the ability of bradykinin to cause calcium-dependent release of glutamate from astrocytes in vitro was investigated. The glutamate transport inhibitor, dihydrokainate, did not block bradykinin-induced glutamate release, and bradykinin did not cause cell swelling. These data exclude the involvement of glutamate transporters or swelling mechanisms as mediating glutamate release in response to bradykinin. α-Latrotoxin (3 nM), a component of black widow spider venom, stimulated calcium-independent glutamate release from astrocytes. Since α-latrotoxin induces vesicle fusion and calcium-independent neuronal neurotransmitter release, our data suggest that astrocytes may release neurotransmitter using a mechanism similar to the neuronal secretory process. © 1995 Federation of European Biochemical Societies.
  • Published In

  • FEBS Letters  Journal
  • Digital Object Identifier (doi)

    Author List

  • Parpura V; Liu F; Brethorst S; Jeftinija K; Jeftinija S; Haydon PG
  • Start Page

  • 266
  • End Page

  • 270
  • Volume

  • 360
  • Issue

  • 3