L-myc cooperates with ras to transform primary rat embryo fibroblasts.

Academic Article

Abstract

  • Recent molecular analysis has revealed that L-myc has several domains of extremely conserved amino acid sequence homology with c-myc and N-myc, suggesting similarity of function. We tested the biologic activity of L-myc by using an expression vector containing a cDNA clone coding for the major open reading frame in the 3.9-kilobase mRNA of L-myc under the control of a strong promoter (Moloney long terminal repeat) and found that L-myc complemented an activated ras gene in transforming primary rat embryo fibroblasts. However, the efficiency of transformation was 1 to 10% of that seen with the c-myc and simian virus 40 (SV40) controls. The L-myc/ras transformants initially grew more slowly than c-myc or SV40 transformants, but once established as continuous cell lines, they were indistinguishable from cell lines derived from c-myc/ras or SV40/ras transfectants as determined by morphology, soft-agar cloning, and tumorigenicity in nude mice.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Birrer MJ; Segal S; DeGreve JS; Kaye F; Sausville EA; Minna JD
  • Start Page

  • 2668
  • End Page

  • 2673
  • Volume

  • 8
  • Issue

  • 6