Asparagine synthetase is a predictive biomarker of L-asparaginase activity in ovarian cancer cell lines

Academic Article

Abstract

  • We recently used RNA interference to show that a negative correlation of L-asparaginase (L-ASP) chemotherapeutic activity with asparagine synthetase (ASNS) expression in the ovarian subset of the NCI-60 cell line panel is causal. To determine whether that relationship would be sustained in a larger, more diverse set of ovarian cell lines, we have now measured ASNS mRNA expression using microarrays and a branched-DNA RNA assay, ASNS protein expression using an electrochemiluminescent immunoassay, and L-ASP activity using an MTS assay on 19 human ovarian cancer cell lines. Contrary to our previous findings, L-ASP activity was only weakly correlated with ASNS mRNA expression; Pearson's correlation coefficients were r = -0.21 for microarray data and r = -0.39 for the branched-DNA RNA assay, with just the latter being marginally statistically significant (P = 0.047, one-tailed). ASNS protein expression measured by liquid-phase immunoassay exhibited a much stronger correlation (r = -0.65; P = 0.0014, one-tailed). We conclude that ASNS protein expression measured by immunoassay is a strong univariate predictor of L-ASP activity in ovarian cancer cell lines. These findings provide rationale for evaluation of ASNS protein expression as a predictive biomarker of clinical L-ASP activity in ovarian cancer. Copyright © 2008 American Association for Cancer Research.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 26434379
  • Author List

  • Lorenzi PL; Llamas J; Gunsior M; Ozbun L; Reinhold WC; Varma S; Ji H; Kim H; Hutchinson AA; Kohn EC
  • Start Page

  • 3123
  • End Page

  • 3128
  • Volume

  • 7
  • Issue

  • 10