Osteopontin adhesion receptors on gingival fibroblasts

Academic Article

Abstract

  • Osteopontin (OPN) promotes attachment and spreading of cells in an RGD dependent fashion, suggesting that OPN interacts with integrins on cell surfaces. Here in, we show that LM‐609, a monoclonal antibody to the αvβ3 integrin (a vitronectin receptor), inhibited OPN‐mediated attachment of gingival fibroblasts. To characterize the cell surface receptors responsible for this interaction, we performed OPN‐sepharose affinity chromatography using detergent extracts of 35S‐methionine or l25I‐surface labeled gingival fibroblasts. Proteins bound to the OPN‐matrix were eluted with EDTA and subjected to SDS‐PAGE under reducing conditions. EDTA eluates from both 125I‐surface labeled and 35Smethionine labeled extracts demonstrated prominent bands in the 90kDa and 50kDa regions, by both autoradiography and fluorography, respectively. These studies suggest that OPN is associated with other cell surface molecules in addition to αvβ3. Furthermore, these as yet to be characterized proteins, may prove to have a stronger affinity for OPN than αvβ3. Copyright © 1995, Wiley Blackwell. All rights reserved
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • D'Errico JA; Sauk JJ; Prince CW; Somerman MJ
  • Start Page

  • 34
  • End Page

  • 41
  • Volume

  • 30
  • Issue

  • 1