Inhibition of prolactin secretion by antiserum to the α- and β-subunits of gonadotropin

Academic Article

Abstract

  • This study was undertaken to determine whether antiserum to the α- and β-subunits of human gonadotropin could affect prolactin secretion from human pituitary cell monolayers. The addition of antiserum to the α-subunit of follicle-stimulating hormone, but not to the α-subunit of luteinizing hormone, at a 1 : 10,000 dilution inhibited basal prolaction secretion. The addition of these antisera failed to inhibit prolactin secretion in the face of 10-5 mol/L gonadotropin-releasing hormone. Coincubation of antiserum to the β-subunit of luteinizing hormone or the β-subunit of follicle-stimulating hormone at a 1 : 10,000 dilution failed to inhibit basal prolactin secretion; however, coincubation of gonadotropin-releasing hormone with antiserum to the β-subunit of either luteinizing hormone or follicle-stimulating hormone significantly inhibited the release of prolactin. These observations led us to conclude that basal and gonadotropin-releasing hormone-mediated prolactin secretion can be affected by gonadotropin subunits. © 1987.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 18845546
  • Author List

  • Blackwell RE; Garrison PN
  • Start Page

  • 863
  • End Page

  • 868
  • Volume

  • 156
  • Issue

  • 4