Role of histone H3 lysine 27 methylation in X inactivation

Academic Article

Abstract

  • The Polycomb group (PcG) protein Eed is implicated in regulation of imprinted X-chromosome inactivation in extraembryonic cells but not of random X inactivation in embryonic cells. The Drosophila homolog of the Eed-Ezh2 PcG protein complex achieves gene silencing through methylation of histone H3 on lysine 27 (H3-K27), which suggests a role for H3-K27 methylation in imprinted X inactivation. Here we demonstrate that transient recruitment of the Eed-Ezh2 complex to the inactive X chromosome (Xi) occurs during initiation of X inactivation in both extraembryonic and embryonic cells and is accompanied by H3-K27 methylation. Recruitment of the complex and methylation on the Xi depend on Xist RNA but are independent of its silencing function. Together, our results suggest a role for Eed-Ezh2-mediated H3-K27 methylation during initiation of both imprinted and random X inactivation and demonstrate that H3-K27 methylation is not sufficient for silencing of the Xi.
  • Published In

  • Science  Journal
  • Digital Object Identifier (doi)

    Author List

  • Plath K; Fang J; Mlynarczyk-Evans SK; Cao R; Worringer KA; Wang H; De la Cruz CC; Otte AP; Panning B; Zhang Y
  • Start Page

  • 131
  • End Page

  • 135
  • Volume

  • 300
  • Issue

  • 5616