Amyloid beta deposition is related to decreased glucose transporter-1 levels and hippocampal atrophy in brains of aged APP/PS1 mice

Academic Article

Abstract

  • The amount of the glucose transporter type-1 (GLUT-1) is decreased in the hippocampus and cerebral cortex of AD patients. In this study we therefore wanted to investigate the causal relationship between β-amyloid (Aβ), GLUT-1 and hippocampal atrophy in the brains of young (8 months) and old (18 months) APP/PS1 mice. Methods: Aβ and GLUT-1 were visualized immunohistochemically. Aβ load, GLUT-1 amount, capillary density and GLUT-1 amount per capillary density were determined in cortical and hippocampal areas using computer-assisted analysis systems. Hippocampal atrophy was determined by calculating the width of the outer molecular layer of the dentate gyrus (DG). Results: In 18-month-old APP/PS1 mice we found a reduced GLUT-1 amount in the hippocampus but no differences in capillary density. The DG of these mice contained the highest level of Aβ in combination with hippocampal atrophy, and a reduced GLUT-1 amount per capillary density. At 8 months, no differences were observed. The highest Aβ deposition was found in the DG, although fourfold less compared to 18-month-old mice. Conclusions: We conclude that the GLUT-1 amount and capillary density in both wild type and transgenic mice decrease due to ageing. Further, a decreased amount of GLUT-1 is caused by decreased GLUT-1 amount/capillary density and not due to a reduced capillary density. We suggest that Aβ load in the hippocampus precedes the reduction of GLUT-1. A certain level of Aβ must be reached in the hippocampus, before it affects GLUT-1 amount/capillary density leading to further impairment of energy metabolism and hippocampal atrophy. © 2007 Elsevier B.V. All rights reserved.
  • Published In

  • Brain Research  Journal
  • Digital Object Identifier (doi)

    Author List

  • Hooijmans CR; Graven C; Dederen PJ; Tanila H; van Groen T; Kiliaan AJ
  • Start Page

  • 93
  • End Page

  • 103
  • Volume

  • 1181
  • Issue

  • 1