Laparoscopic lymph node dissection is routinely performed for diagnosing and staging malignancies in adults; however, reports of this technique used in children have been sparse. We reviewed our experience with laparoscopic lymph node sampling performed in children (mean age 15 years old) at a pediatric oncology center to help determine its efficacy. All patients (n = 8) were suspected of having intraabdominal or pelvic malignancy based on diagnostic imaging. The mean operative time was 158 min (range 55-300 min). The postoperative course was uniformly uneventful, and all patients (except one with histoplasmosis) were fed a regular diet on the day of surgery. There was no immediate or long-term complication in these children and there was no error in diagnosis. Laparoscopic lymph node sampling is minimally invasive and well tolerated. It provides adequate tissue for diagnosis and staging of malignancies in children. Unlike open procedures, adjuvant therapy can be started on the day of surgery. We recommend laparoscopic lymph node sampling as the diagnostic and staging procedure of choice in children with pelvic or intraabdominal lymphadenopathy.