Optimization of quantitative culture assay for human immunodeficiency virus from plasma

Academic Article

Abstract

  • Experimental conditions essential to a simple, reproducible, quantitative human immunodeficiency virus plasma assay were determined. Five parameters were evaluated: length of culture, reproducibility, assay dilution schema, washing procedures, and anticoagulant usage. The recommended quantitative plasma assay utilizes undiluted citrated plasma cultured with peripheral blood mononuclear cells for 14 days with fivefold dilutions and a medium change on day 1 with no washing.
  • Pubmed Id

  • 25801084
  • Author List

  • Lathey JL; Fiscus SA; Rasheed S; Kappes JC; Griffith BP; Elbeik T; Spector SA; Reichelderfer PS
  • Start Page

  • 3064
  • End Page

  • 3067
  • Volume

  • 32
  • Issue

  • 12