Cardiovascular Research

Chapter

Abstract

  • A variety of in vitro techniques have been used to study the normal structure, function, and metabolism of rat myocardium. The rat has been the most widely used species for most of these in vitro techniques, because an adequate amount of tissue is available, function is easily maintained in isolated perfused hearts, and this well-characterized animal model is readily available and relatively inexpensive to procure and maintain. Neonatal myocytes in culture are commonly used for a wide variety of studies, using variations of trypsinization and mincing to prepare the cells. Isolated adult cardiac myocytes have been useful both for studies of myocytes maintained in culture and for morphologic studies of myocytes during normal growth and with a variety of pathologic conditions. Cell isolation is performed by using aortic retrograde perfusion of an isolated heart with oxygenated calcium-free buffer mixtures containing collagenase and other optional enzymes. Fetal rat hearts have been cultured for use in studies of embryonic myocyte growth. The rat myocardium can be used by a number of investigators for preparation of isolated cellular organelles, such as nuclei, mitochondria, ribosomes, or sarcoplasmic reticulum. © 2006 Elsevier Inc. All rights reserved.
  • Authors

    Digital Object Identifier (doi)

    International Standard Book Number (isbn) 13

  • 9780120749034
  • Start Page

  • 773
  • End Page

  • 802