Effect of hyperinsulinemia on whole body and skeletal muscle leucine carbon kinetics in humans.

Academic Article


  • Data documenting the isolated effect of systemic hyperinsulinemia on whole body and skeletal muscle leucine carbon kinetics in humans are limited. Using steady-state [14C]leucine kinetics, 10 normal volunteers were studied in the baseline postabsorptive state and then under euglycemic, hyperinsulinemic (71 +/- 5 microU/ml), and euleucinemic conditions. Systemic hyperinsulinemia resulted in a significant decrease in whole body and forearm leucine rate of appearance (Ra) by 17 and 37%, respectively, (P less than 0.0003, 0.03), without a significant change in the nonoxidized rate of disappearance for either (P = 0.23, 0.66). The baseline contribution of total body skeletal muscle (TBSM) leucine Ra and rate of disappearance (Rd) to whole body leucine Ra and Rd was 27 +/- 6 and 24 +/- 5%, respectively. During hyperinsulinemia TBSM Ra decreased by 34%, whereas whole body Ra decreased by 16%. We conclude that the primary effect of insulin in the whole body and skeletal muscle is to decrease leucine release from protein without a significant effect on leucine incorporation into protein. This antiproteolytic effect of insulin is more pronounced in skeletal muscle than in other tissues in the body.
  • Published In


  • Amino Acids, Bicarbonates, Carbon Radioisotopes, Female, Humans, Hyperinsulinism, Insulin, Kinetics, Leucine, Male, Middle Aged, Models, Biological, Muscles, Radioisotope Dilution Technique, Sodium, Sodium Bicarbonate
  • Digital Object Identifier (doi)

    Pubmed Id

  • 25463515
  • Author List

  • Heslin MJ; Newman E; Wolf RF; Pisters PW; Brennan MF
  • Start Page

  • E911
  • End Page

  • E918
  • Volume

  • 262
  • Issue

  • 6 Pt 1