Falsely incompatible b-cell flow cytometry crossmatch after pronase treatment: A case report

Academic Article


  • © 2015 Elsevier Inc. This report presents a falsely incompatible B cell crossmatch by flow cytometry in a lung transplant recipient. The patient was a 35-year-old Caucasian male with end-stage lung disease secondary to cystic fibrosis whose pretransplantation serologic workup did not disclose the presence of anti-HLA class II antibodies by single antigen bead testing. Unexpectedly, crossmatch of recipient sera with pronase-treated donor lymphocytes resulted in antibody binding to B cells only. The positive reactivity was reproducible in pronase-treated autologous B cells. Recipient sera did not react with nontreated donor or autologous lymphocytes. Herein, we describe our approach to this unexpected crossmatch result and consider the implications of false-positive crossmatch results on transplantation.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 17050534
  • Author List

  • Hart JD; Lutz CT; Jennings CD; May JR; Nelson K; Jacobs S; Hoopes CW
  • Start Page

  • 831
  • End Page

  • 833
  • Volume

  • 47
  • Issue

  • 3