Biological expressions of lymphocyte activation. IV. Concanavalin A activated suppressor cells in mouse mixed lymphocyte reactions

Academic Article


  • Thymus derived lymphocytes (T cells) from mouse spleen, activated in vitro or in vivo with concanavalin A (Con A), suppress proliferative responses of syngenic lymphocytes in mixed lymphocytes reactions (MLR). Replication in vitro was not required for expression of suppressor activity by Con A activated cells and was blocked in MLR by treating suppressor cells with mitomycin C or irradiation. Kinetics of MLR responses and viability of cultures were not altered by addition of activated suppressor cells. The data are consistent with a direct inhibitory effect of suppressor T cells on antigen induced DNA replication. These observations extend a model previously described for the regulation of antibody synthesis by Con A activated T cells to control cell mediated immune responses. This model should be particularly useful in further definition of regulatory T cell subpopulations, and investigation of interactions and relationships between such populations.
  • Authors

    Published In

    Author List

  • Rich RR; Rich SS
  • Start Page

  • 1112
  • End Page

  • 1115
  • Volume

  • 114
  • Issue

  • 3