Cloned human T cells synthetize Ia molecules and can function as antigen presenting cells

Academic Article

Abstract

  • TNP-specific proliferative cloned human T cell lines were investigated for their synthesis and cell surface expression of HLA-DR molecules and for their capacity to function as antigen presenting cells. Utilizing radioactive amino acid precursors for metabolic labeling, these studies demonstrated endogenous synthesis of HLA-DR molecules by cloned T cells, which by two-dimensional gel electrophoresis were similar to HLA-DR molecules expressed by B cells and monocytes. Moreover, when TNP-modified, the irradiated cloned T cells functioned very effectively to stimulate TNP-specific proliferation by cloned responders; when unmodified they were potent stimulators of allogeneic mixed leukocyte responses. Thus, for haptens covalently attached to cell membrane proteins and for allogeneic HLA antigens, Ia+ cloned T cells can function as effectively for antigen presentation and T cell activation as other Ia+ populations to which such properties have been ascribed. © 1984.
  • Authors

    Published In

  • Human Immunology  Journal
  • Digital Object Identifier (doi)

    Author List

  • Brown MF; Cook RG; Van M; Rich RR
  • Start Page

  • 219
  • End Page

  • 228
  • Volume

  • 11
  • Issue

  • 4