Differential amino-terminal anchors for peptide binding to H-2M3a or H- 2Kb and H-2Db1

Academic Article

Abstract

  • We previously established that H-2M3a, the H chain of the maternally transmitted Ag (Mta), is specialized for presentation of N-formylated peptides. We hypothesized that the N-formyl group might prevent or limit the presentation of peptide Ag by H-2K and H-2D molecules. We now show by Mta- and OVA-specific CTL assays, peptide competition, and immunofluorescence analyses that N-formyl modification of four antigenic peptides inhibited their binding by either H-2Kb (OVA(Met258-264), VSVNP52-59, and SVNP324-332) or H2-Db (SVNP324-332, and IVNP366-374). In contrast, N-formyl-OVA(Met258-264) did bind to H2-M3a. The data imply lack of an N-formyl-binding pocket in classical MHC class I molecules and are consistent with a specialized role for H2-M3a in presentation of N- formylated peptides such as derived from intracellular prokaryotic parasites.
  • Authors

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    Author List

  • Shawar SM; Vyas JM; Shen E; Rodgers JR; Rich RR
  • Start Page

  • 201
  • End Page

  • 210
  • Volume

  • 151
  • Issue

  • 1