Polyclonal Rabbit Antisera that Detect the Vpr Protein of SIVSM and SIVMAC on Immunoblots of Purified Virions

Academic Article

Abstract

  • Antisera suitable for detection of SIVSM or SIVMAC Vpr proteins on Western blots of purified virions are currently not available. We have expressed the Vpr protein of SIVSMPBj1.9 in a gst-based prokaryotic expression system and used it to raise polyclonal antisera in rabbits. Two immune sera were obtained that specifically recognized both cell- and virion-associated Vpr protein on immunoblots of three different SIV isolates (SIVSMPBj1.9, SIVMACBK28, and SIVMAC239). Because Vpr is believed to play an important role in HIV/SIV replication and pathogenesis, these reagents will allow the extension of functional analyses of this protein to a broader spectrum of viruses. Both antisera and the gst-Vpr expression plasmid have been submitted to the MAID AIDS Research and Reagent Program and are available to interested investigators. © 1995, Mary Ann Liebert, Inc. All rights reserved.
  • Digital Object Identifier (doi)

    Author List

  • Newman MA; Mcpherson SA; Fletcher TM; Kappes JC; Hahn BH
  • Start Page

  • 405
  • End Page

  • 408
  • Volume

  • 11
  • Issue

  • 3