C/EBPΑ is required for development of dendritic cell progenitors

Academic Article

Abstract

  • Dendritic cells (DCs) are master regulators of the immune system, but molecular regulation of early DC differentiation has been poorly understood. Here, we report that the transcription factor C/EBPa coordinates the development of progenitor cells required for production of multiple categories of DCs. C/EBPa was needed for differentiation from stem/progenitor cells to common DC progenitors (CDPs), but not for transition of CDP to mature DCs. C/EBPa deletion in mature DCs did not affect their numbers or function, suggesting that this transcription factor is not needed for maintenance of DCs in lymphoid tissues. ChIP-seq and microarrays were used to identify candidate genes regulated by C/EBPa and required for DC formation. Genes previously shown to be critical for DC formation were bound by C/EBPa, and their expression was decreased in the earliest hematopoietic compartments in the absence of C/EBPa. These data indicate that C/EBPa is important for the earliest stages of steady-state DC differentiation.
  • Published In

  • Blood  Journal
  • Digital Object Identifier (doi)

    Author List

  • Welner RS; Bararia D; Amabile G; Czibere A; Benoukraf T; Bach C; Wansa KDSA; Ye M; Zhang H; Iino T
  • Start Page

  • 4073
  • End Page

  • 4081
  • Volume

  • 121
  • Issue

  • 20