Involvement of matrix metalloproteinases in human immunodeficiency virus type 1-induced replication by clinical Mycobacterium avium isolates.

Academic Article

Abstract

  • The role of Mycobacterium avium isolates in modulating human immunodeficiency virus type 1 (HIV-1) replication was examined by use of an in vitro, resting T cell system. Two human clinical isolates (serotypes 1 and 4) but not an environmental M. avium isolate (serotype 2) enhanced HIV-1 replication. The M. avium-induced HIV-1 replication was not associated with cell activation or differential cytokine production or utilization. Addition of matrix metalloproteinase (MMP) inhibitors and their in vivo regulators, tissue inhibitors of metalloproteinases-1 and -2, abrogated M. avium-induced HIV-1 replication 80%-95%. The MMP inhibitors did not have any effect on the HIV-1 protease activity, suggesting that they may affect cellular processes. Furthermore, MMP-9 protein was differentially expressed after infection with clinical M. avium isolates and paralleled HIV-1 p24 production. Collectively, these data suggest that M. avium-induced HIV-1 replication is mediated, in part, through the induction of MMP-9.
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    Keywords

  • Animals, Cell Line, Cells, Cultured, HIV Core Protein p24, HIV Protease, HIV-1, Humans, Kinetics, Lymphocyte Depletion, Lymphocytes, Macrophages, Matrix Metalloproteinase 9, Matrix Metalloproteinases, Mycobacterium avium Complex, Mycobacterium avium-intracellulare Infection, Pentoxifylline, Serotyping, Time Factors, Tissue Inhibitor of Metalloproteinase-1, Tissue Inhibitor of Metalloproteinase-2, Tuberculosis, Virus Replication
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    Author List

  • Dezzutti CS; Swords WE; Guenthner PC; Sasso DR; Wahl LM; Drummond AH; Newman GW; King CH; Quinn FD; Lal RB
  • Start Page

  • 1142
  • End Page

  • 1152
  • Volume

  • 180
  • Issue

  • 4