Mycobacterium xenopi multiplies within human macrophages and enhances HIV replication in vitro

Academic Article

Abstract

  • Mycobacterium xenopi can cause opportunistic infections, particularly in persons infected with human immunodeficiency virus type 1 (HIV-1). The primary focus of this effort was to determine if M. xenopi isolates could survive and grow in human peripheral blood macrophage (MΦ), and if these isolates could promote the replication of HIV-1 in vitro. M. xenopi bacilli survived and replicated 10-fold within 48 h in human MΦ while avirulent Mycobacterium smegmatis, did not grow within the MΦ. M. xenopi bacilli when cultured with peripheral blood mononuclear cells enhanced HIV-1 replication 30- and 50-fold with the macrophage-tropic HIV-1Ba-L and 50- and 75-fold with T-cell-tropic strain HIV-1LAI by 6 days post-infection when compared to M. smegmatis. The enhanced HIV replication was associated with increased production of TNF-α. Partial inhibition of HIV-1 induction was observed using a neutralizing anti-TNF-α monoclonal antibody, pentoxifylline, and matrix metalloproteinase (MMP) inhibitor I. Similar mechanisms of pathogenesis among mycobacterial species may help elucidate better treatment approaches in HIV co-infected persons. © 2005 Elsevier Ltd. All rights reserved.
  • Authors

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    Digital Object Identifier (doi)

    Author List

  • Swords WE; Guenthner PC; Birkness KA; Lal RB; Dezzutti CS; Quinn FD
  • Start Page

  • 41
  • End Page

  • 47
  • Volume

  • 40
  • Issue

  • 2