Conversion of the Beckman liquid phase sequencer to a gas-liquid phase sequencer

Academic Article

Abstract

  • As an effective aid to extend the microsequencing capabilities the Beckman protein/peptide sequenator Series 890C has been successfully converted to a gas-liquid system, in which coupling buffer 25% trimethylamine was employed as a gas, and heptafluorobutyric acid as a liquid. The system has been found to be efficient for microsequencing (< 100 pmol). The details of mechanical, plumbing, and other minor changes are described in this paper along with the results of sequencing proteins and peptides, directly and from blots. © 1988.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Bhown AS; Wayland JL; Lynn JD; Bennett JC
  • Start Page

  • 39
  • End Page

  • 51
  • Volume

  • 175
  • Issue

  • 1