Purification and characterization of the gag gene products of avian-type C retroviruses by high-pressure liquid chromatography

Academic Article

Abstract

  • A rapid and efficient method for separating the translational products of the gag gene of avian-type C retroviruses by employing a high-resolution gel permeation column, high-pressure liquid chromatography and volatile solvents has been described. The viral structural components p27, p19, and p15 have been purified in milligram quantities to near homogeneity and in high yields. The purity of the products of separation has been confirmed by amino acid analysis and amino and carboxy terminal amino acid sequence determination, and their molecular integrity was established by immunological methods. © 1981.
  • Authors

    Digital Object Identifier (doi)

    Pubmed Id

  • 9256154
  • Author List

  • Bhown AS; Bennett JC; Mole JE; Hunter E
  • Start Page

  • 128
  • End Page

  • 134
  • Volume

  • 112
  • Issue

  • 1