A comparison of fluorescamine and o-phthaldialdehyde as effective blocking reagents in protein sequence analyses by the Beckman sequencer

Academic Article

Abstract

  • Use of o-phthaldialdehyde to chemically reduce the newly generated amino termini responsible for the progressively increasing background during an extended amino acid sequence analysis in a liquid phase sequencer has been described. The results have been compared with Fluram blocking using apomyoglobin and rabbit C-reactive protein as standard and unknown samples, respectively. © 1983.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Bhown AS; Cornelius TW; Volanakis JE; Bennett JC
  • Start Page

  • 337
  • End Page

  • 340
  • Volume

  • 131
  • Issue

  • 2