Primary Structure of Human J Chain: Isolation and Characterization of Tryptic and Chymotryptic Peptides of Human J Chain

Academic Article

Abstract

  • Human J chain isolated from the plasma of a patient with Waldenstrom's macroglobulinemia was subjected to extended and limited digestion with trypsin and chymotrypsin. The digests were fractionated by combination of column chromatography and high voltage paper electrophoresis. Peptide purity was established by their amino acid analysis and a single amino terminal residue. All the necessary peptides which would provide the total primary structure of molecule were thus obtained. © 1977, American Chemical Society. All rights reserved.
  • Authors

    Published In

  • Biochemistry  Journal
  • Digital Object Identifier (doi)

    Author List

  • Bhown AS; Mole JE; Bennett JC
  • Start Page

  • 3501
  • End Page

  • 3507
  • Volume

  • 16
  • Issue

  • 16