Purification of spfctrin and its subunits by high-performance liquid chromatography

Academic Article


  • Technological and methodological advances in the techniques of structural and biological studies of proteins have reduced the required amount of sample. In conjunction with these advances, high-performance liquid chromatography (HPLC) has emerged as a technique of high utility for the purification of complex molecules. Using a combination of size-exclusion and reversed-phase HPLC and ionic buffers containing sodium dodecyl sulfate, the red cell membrane-associated high-molecular-weight polypeptide spectrin and its subunits have been purified. The system described in this paper is fast, reproducible and quantitative. © 1986.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Bhown AS; Dong JY; Hunter F; Bennett JC
  • Start Page

  • 159
  • End Page

  • 164
  • Volume

  • 369
  • Issue

  • C