Calcium-activated nonselective cationic channel in macula densa cells.

Academic Article


  • Patch-clamp experiments in cell-attached (c/a) and inside-out (i/o) configurations were performed to directly observe ionic channels in lateral membranes of macula densa (MD) cells from rabbit kidney. In the presence of 140 mM KCl in the pipette and normal Ringer solution in the bath, we repeatedly observed in c/a and in i/o configurations a 20- to 23-pS channel with a linear current-voltage (I-V) relationship reversing near 0 mV. Ionic replacement in the bath solution clearly indicated a cationic selectivity but with equal permeability for Na+ and K+. Single-channel kinetics was characterized by higher open probability at positive membrane potentials. In i/o experiments, elimination of bath Ca2+ (
  • Authors


  • Animals, Calcium, Calcium Channel Blockers, Cations, Divalent, Feedback, Physiological, Ion Channels, Juxtaglomerular Apparatus, Kidney Cortex, Nifedipine, Patch-Clamp Techniques, Potassium, Rabbits, Sodium
  • Digital Object Identifier (doi)

    Pubmed Id

  • 23290681
  • Author List

  • Lapointe J-Y; Bell PD; Sabirov RZ; Okada Y
  • Start Page

  • F275
  • End Page

  • F280
  • Volume

  • 285
  • Issue

  • 2