Molecular mechanism of tyrosinase regulation by L-DOPA in hamster melanoma cells

Academic Article


  • Exposure of hamster amelanotic melanoma cells to L-dihydroxyphenylalanine (L-DOPA) resulted in a time dependent increase of cell pigmentation, tyrosinase concentration and activity with peak after 24 hours. Northern blot analysis showed a small but reproducible increase of tyrosinase mRNA after 3 hours and a decrease below the control level after 9 hours. After 24 and 48 hours tyrosinase mRNA was undetectable. It is suggested that L-DOPA or its oxidation products can stimulate intracellular tyrosinase concentration and regulate tyrosinase mRNA level both in positive and negative fashion. © 1991.
  • Published In

  • Life Sciences  Journal
  • Digital Object Identifier (doi)

    Author List

  • Slominski A; Costantino R
  • Start Page

  • 2075
  • End Page

  • 2079
  • Volume

  • 48
  • Issue

  • 21